Keratan sulfates from bovine tracheal cartilage
نویسندگان
چکیده
منابع مشابه
How bovine tracheal cartilage stimulates the immune system
Paradoxically, bovine cartilage stimulates the immune system in resisting cancer and viruses, but suppresses it in rheumatoid diseases. Thus it acts as a “normaliser”. According to Dr. Pridden, “bovine cartilage closely resembles foetal mesenchyme, the primordial tissue from which muscle, bone, tendons, ligaments, skin, fat and bone marrow (the heart of the immune system) all develop.” “The car...
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A glycosidase which attacks corneal keratan sulfate was purified from extracts of Pseudomonas sp. IFO-13309. When corneal keratan sulfate was degraded by the purified enzyme, Sephadex G-50 chromatography indicated the presence of a number of oligosaccharides differing in size and sulfate content. The characterization of two major fractions of the oligosaccharides indicated that the point of enz...
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The assembly of proteoglycan aggregates in chondrocyte cell cultures was examined in pulse-chase experiments with the use of [35S]sulphate for labelling. Rate-zonal centrifugation in linear sucrose density gradients (10-50%, w/v) was used to separate the aggregated proteoglycans from monomers and to assess the size of the newly formed aggregates. The proportion of aggregates stabilized by link ...
متن کاملCharacterization of the keratan sulphate proteoglycans from bovine corneal stroma.
The keratan sulphate proteoglycans that can be prepared from bovine corneal stroma [Axelsson & Heinegård (1975) Biochem. J. 145, 491-500] were characterized by gel chromatography, gel electrophoresis and analytical ultracentrifugation in associative (0.6 M-NaCl) and dissociative (6M-guanidinum chloride) solvents. The proteoglycans aggreagated at low salt concentrations and pH. The weight-averag...
متن کاملDistribution of keratan sulfate in cartilage proteoglycans.
After chondroitinase digestion of bovine nasal and tracheal cartilage proteoglycans, subsequent treatment with trypsin or trypsin followed by chymotrypsin yielded two major types of polypeptide-glycosaminoglycan fragments which could be separated by Sepharose 6B chromatography. One fragment, located close to the hyaluronic acid-binding region of the protein core, had a high relative keratan sul...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2000
ISSN: 0014-2956
DOI: 10.1046/j.1432-1327.2000.01374.x